What You'll Need
- Lyophilized peptide vial
- Bacteriostatic water (BAC water)
- Alcohol swabs
- Sterile syringes (insulin syringes work well)
Step-by-Step Instructions
Step 1: Gather Materials
Ensure you have all materials ready and your workspace is clean. Wash your hands thoroughly before beginning.
Step 2: Clean the Vial Tops
Use an alcohol swab to clean the rubber stopper on both the peptide vial and the bacteriostatic water vial. Allow to air dry.
Step 3: Calculate the Amount of BAC Water
The amount of bacteriostatic water you add determines your concentration. Common reconstitution volumes:
- 5mg peptide + 2.5ml BAC water = 2mg/ml (or 200mcg per 0.1ml)
- 5mg peptide + 2ml BAC water = 2.5mg/ml (or 250mcg per 0.1ml)
- 10mg peptide + 2ml BAC water = 5mg/ml (or 500mcg per 0.1ml)
Step 4: Add BAC Water Slowly
Draw up the calculated amount of bacteriostatic water. Insert the needle into the peptide vial and let the water drip down the side of the vial. Do NOT spray directly onto the powder.
⚠️ Important: Never shake the vial. Peptides are fragile and shaking can damage them. Instead, gently swirl or let it sit until dissolved.
Step 5: Allow to Dissolve
Let the vial sit for a few minutes. You can gently roll it between your palms to help dissolve the powder. The solution should become clear.
Step 6: Store Properly
Once reconstituted, store the peptide in the refrigerator (2-8°C). Most reconstituted peptides remain stable for 3-4 weeks when stored properly.
Pro Tip: Always use bacteriostatic water, not sterile water. BAC water contains 0.9% benzyl alcohol which prevents bacterial growth and extends the peptide's shelf life.